Problems inherent in obtaining the alkaline phosphatase reaction.
نویسندگان
چکیده
منابع مشابه
Alkaline phosphatase for immunocytochemical labelling: problems with endogenous enzyme activity.
Alkaline phosphatase may be used as a label for immunocytochemistry and can be demonstrated in tissue sections using the single step naphthol phosphate method. Endogenous enzyme activity may not be destroyed by fixation in formalin, formol alcohol, Carnoy's or Baker's solutions and should be inhibited before results are assessed. Either Bouin's solution or periodic acid followed by potassium bo...
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Background: The present study was carried out on the human population of Kashmir valley to evaluate the status of the biochemical parameters of infected population. Materials and Methods: Blood samples were collected from 514 individuals, 298 (57.97%) males and 216 (42.02%) females, 187 (36.38%) were found sero-positive for human toxocariasis. Results: Alkaline phosphatase level was found highe...
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The aim of this study was to Investigate the toxic effects of chromium and cadmium on the activity of two isoenzymes of alkaline phosphatase in vitro and in vivo experiments. Oral administration and peritoneal injection of CdCl2 or CrCl3 in rats reduced serum alkaline phosphatase by 21.5℅ and 25.5℅ respectively. At the same time high molecular weight alkaline phosphatase was elevated by 138℅ a...
متن کاملThe kinetics of alkaline phosphatase.
The repressible alkaline phosphatase of Escherichia coli (orthophosphoric monoester phosphohydrolase, EC 3.1.3.1) promises to play a role in biochemical genetics comparable to that played by fl-galactosidase (l-5). It is also being used increasingly for class experiments. The evaluation of the important characteristic kinetic constants for this enzyme, however, poses certain technical difficult...
متن کاملA model of the transition state in the alkaline phosphatase reaction.
A high resolution crystal structure of Escherichia coli alkaline phosphatase in the presence of vanadate has been refined to 1.9 A resolution. The vanadate ion takes on a trigonal bipyramidal geometry and is covalently bound by the active site serine nucleophile. A coordinated water molecule occupies the axial position opposite the serine nucleophile, whereas the equatorial oxygen atoms of the ...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1980
ISSN: 0022-1554,1551-5044
DOI: 10.1177/28.1.6153197